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Authentication of Chinese medicinal materials by DNA technology

  • 229 Pages
  • 4.34 MB
  • 6956 Downloads
  • English

World Scientific , River Edge, N.J
Materia medica -- China -- Analysis., DNA fingerprinting., Medicine, Chinese -- Formulae, receipts, prescriptions., Materia Medica -- analysis., Genetic Techniques., Pharmaceutical Preparations -- standards., Drugs, Chinese Herbal -- standards., Drug Contamination -- prevention & control., Medicine, Chinese Traditional., Species Specifi

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Other titlesChinese medicinal materials by DNA technology
Statement[edited by] P.C. Shaw, J. Wang, P.P.-H. But.
ContributionsShaw, P.-C., Wang, J., But, Paul P. H., Chinese University of Hong Kong. Chung Chi College.
Classifications
LC ClassificationsRS180.C5 A95 2002
The Physical Object
Paginationxii, 229 p. :
ID Numbers
Open LibraryOL3670563M
ISBN 109810246218
LC Control Number2002727064
OCLC/WorldCa50758648

Authentication of Chinese Medicinal Materials by DNA Technology: Medicine & Health Science Books @ ed by:   The contents of the book are divided into four parts. The first part reviews the current status of molecular authentication of Chinese medicinal materials.

The second part describes the equipment and materials required for the work. In the third part, researchers in the field present the principles and step-by-step protocols for a wide spectrum of techniques used in the molecular authentication of TCM. DNA molecules offer a more definitive means of authentication, as genetic composition is unique for each taxon and is less affected by age, physiological conditions and environmental factors.

This book introduces and evaluates the molecular techniques that have been developed to authenticate Chinese medicinal materials. Authentication of Chinese Medicinal Materials by Polymerase Chain Reaction Using Arbitrarily Chosen Primers. Hui Cao, Paul Pui-Hay But, Fai-Ngor Ngan, Jun Wang; and ; Pang-Chui Shaw.

Traditional Chinese medicine (TCM) has been used for centuries in the Orient to relieve or treat diseases.

Its efficacy is now attracting worldwide attention. Chinese medicinal materials are traditionally identified by their organoleptic character. [and others] --Methodology and equipment for general molecular techniques / Forrest Chung-Fai Yau and Fai-Ngor Ngan --Authentication of Chinese medicinal materials by polymerase chain reaction using arbitrarily chosen primers / Hui Cao [and others] --Authentication of the components from a mixture of herbal materials / Kur-Ta Cheng.

Department of Biochemistry and Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong Search for more papers by this author Wai-Yan Ha.

The first part reviews the current status of molecular authentication of Chinese medicinal materials. The second part describes the equipment and materials required for the work. In the third part, researchers in the field present the principles and step-by-step protocols for a wide spectrum of techniques used in the molecular authentication of TCM.

Department of Biochemistry and Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong Search for more papers by this author Jun Wang.

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provides an informative introduction to DNA me thods for authentication of Chinese medicinal materials. Technical feat ures and examples of the methods ba sed on sequencing, hybridization and.

Department of Biochemistry and Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong Search for more papers by this author Fai-Ngor Ngan. Abstract. Correctly identified medicinal materials are the premise of research and applications on Chinese medicine.

Details Authentication of Chinese medicinal materials by DNA technology FB2

DNA authentication is an accurate method for routine identification of medicinal : Ka-Lok Wong, Yat-Tung Lo, Pang-Chui Shaw. DNA technology provides a powerful tool to complement chemical analyses for authentication of Chinese medicinal plants and to ensure that herbal materials are not contaminated with ineffective or potentially harmful substitutes or by: Molecular Authentication of the Chinese Herb Huajuhong and Related Medicinal Material by DNA Sequencing and ISSR Markers How we measure 'reads' A.

cal authentication as related species oftenpossess similar features. With the improvements in molecular biotechnol-ogyand plant genetics in the past decades, genet-ic tools are considered to provide more reliability for the authentication of herbal materials at the DNA level.

Thus various DNA-based molecular marker techniques are meanwhile. As adulterated and substituted Chinese medicinal materials are common in the market, therapeutic effectiveness of such materials cannot be guaranteed. Identification at species- strain- and locality-levels, therefore, is required for quality assurance/control of Chinese by: DNA based identification of medicinal materials in Chinese patent medicines Article (PDF Available) in Scientific Reports December with Reads How we measure 'reads'.

Editorial Board of Chinese Plant Annals Chinese Academy of Sciences () Chinese Plant Annals, Vol. Beijing: Science Press, 75– Lau, DT, Shaw, PC, Wang, J and But, PP () Authentication of medicinal Dendrobium species by the internal transcribed spacer of ribosomal by:   DNA methods are suitable for identifying Chinese medicinal materials because genetic composition is unique for each individual irrespective of the physical forms of samples, and is less affected by age [19], physiological conditions, environmental factors [20–22], harvest [19], storage and processing [23–31].Cited by: Molecular techniques provide alternative means to conventional organoleptic and chemical authentication methods and are often more superior in accuracy, sensitivity, resolution and reproducibility.

Since the early s, a number of molecular techniques have been developed to identify traditional medicinal materials based on DNA Cited by: 8. A total of raw herbal samples representing medicinal species were collected from the 7 primary markets in China.

The advantages and limitations of DNA barcoding for the authentication of complex TCM materials by using the TCMD database are also discussed. Additional details are described in subsequent by:   For example, Chinese medicinal materials are regulated by the Department of Health in Taiwan [ 6 ].

Herbal manufacturers must label their product with the names of the herbs in the product, and products without such labels are prohibited from entering by: Shaw PC, et al.

Authentication of Chinese Medicinal Materials by DNA Technology. Journal of Ethnopharmacology. ; Tomlinson B, et al. Toxicity of complementary therapies: an eastern perspective.

Journal of Clinical Pharmacology. ; –Author: Hui Guo, Siyang Jin, Han Liu, Zhenyue Wang. Medicinal plants have been used worldwide for centuries to maintain health and to treat diseases, more so chronic diseases.

However, adulteration and use of spurious materials as substitutes have become a major concern for users and industry for reasons of safety and efficacy. Therefore, authentication of medicinal plants is of utmost by: It also enjoys a growing popularity both in eastern and western countries nowadays rather than abandoned by the highly developed science and technology.

According to different processing technology, TCM can be divided into Chinese medicinal raw materials, prepared slices and Chinese patent medicines (CPM).Cited by: Chinese and Botanical Medicines: Traditional Uses and Modern Scientific Approaches is a classroom-tested book that contains a balance of chemistry, the history of Traditional Chinese Medicine (TCM), and the theory and practice of a modern TCM practitioner.

This distinct book reviews the scientific methods for collecting data and supporting evidence for the efficacy and safety of Chinese drugs Author: Raymond Cooper, Chun-Tao Che, Daniel Kam-Wah Mok, Charmaine Wing-Yee Tsang.

Description Authentication of Chinese medicinal materials by DNA technology FB2

DNA manipulation techniques developed for molecular biotechnology have been adapted to the authentication of herbs in recent years.

These techniques comprise of the molecular markers, sequencing of specific genes, and sophisticated hybridization setups such as DNA microarrays. The DNA barcode for species identification is a global method that enables the identification of TCM to form a unified international standard.

It can greatly improve the identification level of Chinese medicinal materials by establishing a Chinese herbal medicine DNA barcode by: – – Chinese Journal of Natural Medicines15(7):  Chinese Journal of Natural Medicines •Research Articles• Identification of processed Chinese medicinal materials using DNA mini-barcoding SONG Ming1, 2, 3, DONG Gang-Qiang3Δ, ZHANG Ya-Qin1, 2, LIU Xia1, 2*, SUN Wei1* 1 Institute of Chinese Materia Medica, China Academy of Chinese Medical Cited by: 4.

Chinese medicinal materials may be authenticated by molecular identification. As a definitive approach to molecular identification of medicinal materials, forensically informative nucleotide sequencing (FINS) comprises four steps, namely (1) DNA extraction from biological samples, (2) selection and amplification of a specific DNA fragment, (3) determination of the sequence of the amplified DNA Cited by:.

Financial support: Nationally Natural Science Foundation of China (No. ) and the National Infrastructure of Natural Resources for Science and Technology (No. DKA). Keywords: Angelica sinensis, internal transcribed spacer, molecular authentication, specific primers, traditional Chinese by: DNA Barcoding Chinese Medicine has been used for centuries and is still widely used today.

However, substitutes and adulterants of medicinal materials are often introduced either intentionally or With the advance in molecular technology, herb authentication based on Deoxyribonucleic Acid.

2 – 5 December Worldwide Renowned Scientists Gathered in International Conference for DNA Authentication of Herbal Material. Over scientists and personnel from herbal industry and regulatory agencies from 10 countries or areas attended the 2nd International Conference on DNA Technology for Authentication, Quality Control and Conservation of Herbal Material from 2 .